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eclipse structured illumination microscopy (sim) imaging system  (Nikon)


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    Nikon eclipse structured illumination microscopy (sim) imaging system
    Localization of RPA-single stranded DNA in WT, rfa1-t11 , rad52* and rfa1-t11 rad52* cells. ( A ) Immunofluorescence analysis of a meiotic nuclear spread of WT, rfa1-t11 , rad52* and rfa1-t11 rad52* . The cells were stained for anti-Rfa1 (Red) and anti-HA (Green). The scale bar represents 2.5 μm. (i–iv) The boxed regions at the bottom of each panel are magnified images. The scale bar of magnified images represents 1 μm. ( B ) Representative <t>structured</t> <t>illumination</t> <t>microscopy</t> <t>(SIM)</t> images of WT (4 h), rfa1-t11 (4 h), rad52* (5 h) and rfa1-t11 rad52* (6 h) mutant chromosomes. The cells were stained for Rec8 (Green) and Rfa1 (Red). The scale bar represents 2.5 μm. Enlarged images of the boxed regions are shown to the right of each panel. ( C ) Immunofluorescence analysis of a meiotic nuclear spread in zip1Δ , zip1Δ rfa1-t11 , zip1Δ rad52* and zip1Δ rfa1-t11 rad52* . The cells were stained for Rec8 (Green) and Rfa1 (Red). ( D ) Number of RPA foci per nucleus in the WT, spo11-Y135F , rfa1-t11 , rad52* and rfa1-t11 rad52* strains. Data are presented as the mean ± SD ( N ≥ 20).
    Eclipse Structured Illumination Microscopy (Sim) Imaging System, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse structured illumination microscopy (sim) imaging system/product/Nikon
    Average 90 stars, based on 1 article reviews
    eclipse structured illumination microscopy (sim) imaging system - by Bioz Stars, 2026-04
    90/100 stars

    Images

    1) Product Images from "RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination"

    Article Title: RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkae083

    Localization of RPA-single stranded DNA in WT, rfa1-t11 , rad52* and rfa1-t11 rad52* cells. ( A ) Immunofluorescence analysis of a meiotic nuclear spread of WT, rfa1-t11 , rad52* and rfa1-t11 rad52* . The cells were stained for anti-Rfa1 (Red) and anti-HA (Green). The scale bar represents 2.5 μm. (i–iv) The boxed regions at the bottom of each panel are magnified images. The scale bar of magnified images represents 1 μm. ( B ) Representative structured illumination microscopy (SIM) images of WT (4 h), rfa1-t11 (4 h), rad52* (5 h) and rfa1-t11 rad52* (6 h) mutant chromosomes. The cells were stained for Rec8 (Green) and Rfa1 (Red). The scale bar represents 2.5 μm. Enlarged images of the boxed regions are shown to the right of each panel. ( C ) Immunofluorescence analysis of a meiotic nuclear spread in zip1Δ , zip1Δ rfa1-t11 , zip1Δ rad52* and zip1Δ rfa1-t11 rad52* . The cells were stained for Rec8 (Green) and Rfa1 (Red). ( D ) Number of RPA foci per nucleus in the WT, spo11-Y135F , rfa1-t11 , rad52* and rfa1-t11 rad52* strains. Data are presented as the mean ± SD ( N ≥ 20).
    Figure Legend Snippet: Localization of RPA-single stranded DNA in WT, rfa1-t11 , rad52* and rfa1-t11 rad52* cells. ( A ) Immunofluorescence analysis of a meiotic nuclear spread of WT, rfa1-t11 , rad52* and rfa1-t11 rad52* . The cells were stained for anti-Rfa1 (Red) and anti-HA (Green). The scale bar represents 2.5 μm. (i–iv) The boxed regions at the bottom of each panel are magnified images. The scale bar of magnified images represents 1 μm. ( B ) Representative structured illumination microscopy (SIM) images of WT (4 h), rfa1-t11 (4 h), rad52* (5 h) and rfa1-t11 rad52* (6 h) mutant chromosomes. The cells were stained for Rec8 (Green) and Rfa1 (Red). The scale bar represents 2.5 μm. Enlarged images of the boxed regions are shown to the right of each panel. ( C ) Immunofluorescence analysis of a meiotic nuclear spread in zip1Δ , zip1Δ rfa1-t11 , zip1Δ rad52* and zip1Δ rfa1-t11 rad52* . The cells were stained for Rec8 (Green) and Rfa1 (Red). ( D ) Number of RPA foci per nucleus in the WT, spo11-Y135F , rfa1-t11 , rad52* and rfa1-t11 rad52* strains. Data are presented as the mean ± SD ( N ≥ 20).

    Techniques Used: Immunofluorescence, Staining, Microscopy, Mutagenesis



    Similar Products

    eclipse structured illumination microscopy (sim) imaging system  (Nikon)
    90
    Nikon eclipse structured illumination microscopy (sim) imaging system
    Localization of RPA-single stranded DNA in WT, rfa1-t11 , rad52* and rfa1-t11 rad52* cells. ( A ) Immunofluorescence analysis of a meiotic nuclear spread of WT, rfa1-t11 , rad52* and rfa1-t11 rad52* . The cells were stained for anti-Rfa1 (Red) and anti-HA (Green). The scale bar represents 2.5 μm. (i–iv) The boxed regions at the bottom of each panel are magnified images. The scale bar of magnified images represents 1 μm. ( B ) Representative <t>structured</t> <t>illumination</t> <t>microscopy</t> <t>(SIM)</t> images of WT (4 h), rfa1-t11 (4 h), rad52* (5 h) and rfa1-t11 rad52* (6 h) mutant chromosomes. The cells were stained for Rec8 (Green) and Rfa1 (Red). The scale bar represents 2.5 μm. Enlarged images of the boxed regions are shown to the right of each panel. ( C ) Immunofluorescence analysis of a meiotic nuclear spread in zip1Δ , zip1Δ rfa1-t11 , zip1Δ rad52* and zip1Δ rfa1-t11 rad52* . The cells were stained for Rec8 (Green) and Rfa1 (Red). ( D ) Number of RPA foci per nucleus in the WT, spo11-Y135F , rfa1-t11 , rad52* and rfa1-t11 rad52* strains. Data are presented as the mean ± SD ( N ≥ 20).
    Eclipse Structured Illumination Microscopy (Sim) Imaging System, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eclipse structured illumination microscopy (sim) imaging system/product/Nikon
    Average 90 stars, based on 1 article reviews
    eclipse structured illumination microscopy (sim) imaging system - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Localization of RPA-single stranded DNA in WT, rfa1-t11 , rad52* and rfa1-t11 rad52* cells. ( A ) Immunofluorescence analysis of a meiotic nuclear spread of WT, rfa1-t11 , rad52* and rfa1-t11 rad52* . The cells were stained for anti-Rfa1 (Red) and anti-HA (Green). The scale bar represents 2.5 μm. (i–iv) The boxed regions at the bottom of each panel are magnified images. The scale bar of magnified images represents 1 μm. ( B ) Representative structured illumination microscopy (SIM) images of WT (4 h), rfa1-t11 (4 h), rad52* (5 h) and rfa1-t11 rad52* (6 h) mutant chromosomes. The cells were stained for Rec8 (Green) and Rfa1 (Red). The scale bar represents 2.5 μm. Enlarged images of the boxed regions are shown to the right of each panel. ( C ) Immunofluorescence analysis of a meiotic nuclear spread in zip1Δ , zip1Δ rfa1-t11 , zip1Δ rad52* and zip1Δ rfa1-t11 rad52* . The cells were stained for Rec8 (Green) and Rfa1 (Red). ( D ) Number of RPA foci per nucleus in the WT, spo11-Y135F , rfa1-t11 , rad52* and rfa1-t11 rad52* strains. Data are presented as the mean ± SD ( N ≥ 20).

    Journal: Nucleic Acids Research

    Article Title: RPA interacts with Rad52 to promote meiotic crossover and noncrossover recombination

    doi: 10.1093/nar/gkae083

    Figure Lengend Snippet: Localization of RPA-single stranded DNA in WT, rfa1-t11 , rad52* and rfa1-t11 rad52* cells. ( A ) Immunofluorescence analysis of a meiotic nuclear spread of WT, rfa1-t11 , rad52* and rfa1-t11 rad52* . The cells were stained for anti-Rfa1 (Red) and anti-HA (Green). The scale bar represents 2.5 μm. (i–iv) The boxed regions at the bottom of each panel are magnified images. The scale bar of magnified images represents 1 μm. ( B ) Representative structured illumination microscopy (SIM) images of WT (4 h), rfa1-t11 (4 h), rad52* (5 h) and rfa1-t11 rad52* (6 h) mutant chromosomes. The cells were stained for Rec8 (Green) and Rfa1 (Red). The scale bar represents 2.5 μm. Enlarged images of the boxed regions are shown to the right of each panel. ( C ) Immunofluorescence analysis of a meiotic nuclear spread in zip1Δ , zip1Δ rfa1-t11 , zip1Δ rad52* and zip1Δ rfa1-t11 rad52* . The cells were stained for Rec8 (Green) and Rfa1 (Red). ( D ) Number of RPA foci per nucleus in the WT, spo11-Y135F , rfa1-t11 , rad52* and rfa1-t11 rad52* strains. Data are presented as the mean ± SD ( N ≥ 20).

    Article Snippet: Super-resolution images of chromosome spread were acquired using a Nikon Eclipse Structured Illumination Microscopy (SIM) imaging system equipped with an EM CCD camera iXon897 (100× Plan Apochromat lens, 1.49 NA).

    Techniques: Immunofluorescence, Staining, Microscopy, Mutagenesis